Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery

RNA-only multi-input microRNA classifier circuit differentiates between HeLa, HEK293 and MCF7 cells

Synthetic regulatory circuits encoded in RNA rather than DNA could provide a means to control cell behavior while avoiding potentially harmful genomic integration in therapeutic applications.  Weiss Lab has created post-transcriptional circuits using RNA-binding proteins, which can be wired in a plug-and-play fashion to create networks of higher complexity.  The circuits function in mammalian cells when encoded in modified mRNA or self-replicating RNA.

Nature Biotechnology

 

Top